01 Project initiation

02 Biomarker discovery

03 Raw material development

04 Single molecule kits development

05 Kit performance assessment and validation

06 Clinical transformation and application

Background check, key indicator inputs

Development purpose
Development endpoint
Assessment indicators
Technical requirements
Outpu

Proteomic assay

Monoclonal/polyclonal antibodies preparation

Antigen preparation
Antibodies preparation&assessment
Paired antibody assessment

Bio-labeling, reagent optimization

Magnetic bead screening
Buffer system
Anti-interference system
Concentration optimization

Output of test results in accordance with technical requirements

Sensitivity
Specificity
Detection range
Anti-interference ability
Stability
Registered use output complete information

Clinical sample validation and model construction